RNA modifications in diverse cellular RNAs, often collectively termed the “epitranscriptome,” have emerged as important regulators of most aspects of gene expression ( Roundtree et al. YTHDC2 was recently found to promote a “fast-track” expression program for specific mRNAs, and our data suggest that YTHDC2 accomplishes this by recruitment of the RNA degradation machinery to regulate the stability of m 6A-containing mRNAs and by utilizing its distinct RNA-binding domains to bridge interactions between m 6A-containing mRNAs and the ribosomes to facilitate their efficient translation. We show that the YTH and R3H domains contribute to the binding of YTHDC2 to cellular RNAs, and using crosslinking and analysis of cDNA (CRAC), we reveal that YTHDC2 interacts with the small ribosomal subunit in close proximity to the mRNA entry/exit sites. The YTH domain of YTHDC2 preferentially binds m 6A-containing RNAs via a conserved hydrophobic pocket, whereas the ankyrin repeats mediate an RNA-independent interaction with the 5′–3′ exoribonuclease XRN1. In contrast to the other YTH domain-containing proteins, YTHDC2 has several defined domains and here, we have analyzed the contribution of these domains to the RNA and protein interactions of YTHDC2. Several YTH domain-containing proteins have been identified as m 6A readers that regulate the splicing, translation, or stability of specific mRNAs. While m 6As can have direct effects on the structure, maturation, or translation of mRNAs, such modifications can also influence the fate of RNAs via proteins termed “readers” that specifically recognize and bind modified nucleotides. And if you have a favorite that we didn't include here (there are many of them out there, no offense intended) - share with your friends.N 6-methyladenosine (m 6A) modifications in RNAs play important roles in regulating many different aspects of gene expression. If you've tried some of these RSS options, let us know what you thought. And in the wake of Google's announcement, almost all of the services listed here are posting special "How to import your subscriptions from Reader" instructions. If you're wondering how you might export your RSS subscriptions to another reader, Google says you can use its Google Takeout service to download.
For instance, NewsBlur allows you to subscribe to receive stories from as many as 64 sites for free if you want more than that, you'll need an upgraded account, which costs about $2 a month. Many of the RSS services that might replace Reader are free - up to a point. In the future, another option might come from Digg the link-sharing community says it's building its own reader service. Similarly, Taptu offers a sharp, clean user experience, and it offers versions for Nook and Blackberry users, as well. In a similar vein, there's Flipboard, but the smooth design of that service is only available on smartphones and tablets. Or you can go in the other direction, visually at least, and try out Netvibes, which has a glossy, customizable look. Not far behind was The Old Reader, which is reportedly based on an older design of Google Reader. And it should work well in both browsers and smartphones' operating systems.Īt the website Replace Reader, which uses tweets to tally votes for Google replacements, the Feedly service was in the lead, with NewsBlur in second place. In the discussions, a consensus of criteria emerged, calling for a simple and reliable RSS reader that serves up headlines, stories and news digests in an efficient design, without advertising. Some folks will likely do that - but many people are also looking for new RSS readers, and they're comparing notes about possible contenders. Many observers see the move to retire Reader as Google prodding its users to engage more fully with Google+, the social network. That decline has been attributed to people relying on social media sites such as Twitter and Facebook both to stay current on headlines, and to stay connected with a community of people they're interested in - two strengths that helped Google Reader's fast initial growth. Google says that while the service it began in 2005 "has a loyal following, over the years usage has declined." The complaints are still rolling in - as of Friday afternoon, a petition at had gathered more than 100,000 signatures, protesting the move Google announced on its blog Wednesday.
To illustrate the level of dependency they've come to feel, some are comparing the move to Google abandoning search. Google's announcement this week that it will kill its Reader product on July 1 prompted moans of despair from those who rely on the free RSS service to monitor headlines. Google's announcement this week that it would discontinue its Reader after the end of June has left loyal users angry - and scrambling for replacements.